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bioRxiv · 10.1101/2021.01.19.427283

Oncogenic RAS instructs morphological transformation of human epithelia via differential tissue mechanics.

Nyga, A.·Munoz, J.·Dercksen, S.·Fornabaio, G.·Uroz, M.·Trepat, X.·Baum, B.·Matthews, H. K.·Conte, V.
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Abstract

The RAS proto-oncogene is a critical regulator of cell state, morphology and mechanics, and plays a key role in cancer progression. Here, by using a human epithelial model in vitro, we ask how morpho-mechanical changes driven by oncogenic RAS activation at the level of individual cells are collectively integrated to drive changes in tissue behaviour. We found that the uniform oncogenic expression of HRAS.V12 in confined epithelial monolayers causes reproducible changes in the structure and organization of the tissue, which acquires a transitory bilayered morphology. RAS-driven bilayering associates with reproducible layer-specific differences in cell-cell contractility and cell-matrix forces. These drive the initially flat tissues to form three-dimensional structures mimicking some of the behaviours seen in human cancers. Our findings establish a physical mechanism of cellular collectives through which uniform expression of RAS can be interpreted differently in different places of the same tissue to regulate its physiological and pathological morphology.

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Nyga, A., Munoz, J., Dercksen, S., Fornabaio, G., Uroz, M., Trepat, X., Baum, B., Matthews, H. K., Conte, V.. 2021-01-20. Oncogenic RAS instructs morphological transformation of human epithelia via differential tissue mechanics.. https://doi.org/10.1101/2021.01.19.427283

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