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bioRxiv · 10.1101/2021.01.19.427248

Analysis of HIV Reservoirs in Cellular Conjugates from Peripheral Blood

Abstract

Defining distinctive attributes of HIV-infected cells will inform development of HIV cure-directed therapies. Prior ex vivo studies of blood and tissue have suggested that some HIV-infected CD4 T cells are found in conjugates with other cell types. Here, we analyzed levels and sequences of HIV nucleic acids in sorted cellular conjugates from PBMC. Compared to single CD4 T cells, conjugates containing CD4 T cells showed no enrichment for HIV DNA or RNA. However, in several HIV controllers, HIV DNA sequences from sorted conjugates were enriched for sequences closely related to plasma viruses. In ART-treated people, although subgenomic HIV DNA sequences in sorted conjugates and single cells were genetically intermingled, intact proviruses were more frequent in whole blood cells than in magnetically-purified CD4 T cells. We conclude that some HIV-infected cells have attributes that predict preferential loss during sample processing, and that may also reflect vulnerability to therapeutic targeting in vivo.

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Boritz, E., Perez, L., Crespo-Velez, D., Lee, M., Zakaria, S., Poole, A., Bell, J., Migueles, S., Chun, T.-W., Moir, S., Maldarelli, F.. 2021-01-21. Analysis of HIV Reservoirs in Cellular Conjugates from Peripheral Blood. https://doi.org/10.1101/2021.01.19.427248

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