Large-scale cellular-resolution imaging of neural activity in freely behaving mice

Leman, D. P.; Chen, I. A.; Bolding, K. A.; Tai, J.; Wilmerding, L. K.; Gritton, H. J.; Cohen, Y.; Yen, W. W.; Perkins, L. N.; Liberti, W. A.; Kilic, K.; Han, X.; Cruz-Martin, A.; Gardner, T. J.; Otchy, T. M.; Davison, I. G.

doi:10.1101/2021.01.15.426462

bioRxiv · 10.1101/2021.01.15.426462

Large-scale cellular-resolution imaging of neural activity in freely behaving mice

Leman, D. P.·Chen, I. A.·Bolding, K. A.·Tai, J.·Wilmerding, L. K.·Gritton, H. J.·Cohen, Y.·Yen, W. W.·Perkins, L. N.·Liberti, W. A.·Kilic, K.·Han, X.·Cruz-Martin, A.·Gardner, T. J.·Otchy, T. M.·Davison, I. G.

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Abstract

Miniaturized microscopes for head-mounted fluorescence imaging are powerful tools for visualizing neural activity during naturalistic behaviors, but the restricted field of view of first-generation miniscopes limits the size of neural populations accessible for imaging. Here we describe a novel miniaturized mesoscope offering cellular-resolution imaging over areas spanning several millimeters in freely moving mice. This system enables comprehensive visualization of activity across entire brain regions or interactions across areas.

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Leman, D. P., Chen, I. A., Bolding, K. A., Tai, J., Wilmerding, L. K., Gritton, H. J., Cohen, Y., Yen, W. W., Perkins, L. N., Liberti, W. A., Kilic, K., Han, X., Cruz-Martin, A., Gardner, T. J., Otchy, T. M., Davison, I. G.. 2021-01-17. Large-scale cellular-resolution imaging of neural activity in freely behaving mice. https://doi.org/10.1101/2021.01.15.426462

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