Synthesis, Characterization, and Nanodisc formation of Non-ionic Polymers

Ravula, T.; Ramamoorthy, A.

doi:10.1101/2021.01.11.426267

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bioRxiv · 10.1101/2021.01.11.426267

Synthesis, Characterization, and Nanodisc formation of Non-ionic Polymers

Ravula, T.·Ramamoorthy, A.

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Abstract

Despite lipid-nanodiscs are increasingly used in the structural studies of membrane proteins, drug delivery and other applications, the interaction between the nanodisc-belt and the protein to be reconstituted is a major limitation. To overcome this limitation and to further broaden the scope of nanodiscs, a family of non-ionic amphiphilic polymers synthesized by hydrophobic functionalization of fructo-oligosaccharides/inulin is reported. We show the stability of lipid-nanodiscs formed by these polymers against pH and divalent metal ions, and their magnetic-alignment properties. The reported results also demonstrate that the non-ionic polymers extract membrane proteins with unprecedented efficiency.

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Ravula, T., Ramamoorthy, A.. 2021-01-11. Synthesis, Characterization, and Nanodisc formation of Non-ionic Polymers. https://doi.org/10.1101/2021.01.11.426267

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