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bioRxiv · 10.1101/2021.01.11.426231

A counterintuitive method for slowing down an analyte in a nanopore and its application to polymer sequencing

Sampath, G.
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Abstract

An unresolved problem in nanopore sensing is the high translocation speed ([~]10-100 monomers/s) of an analyte (nucleotide, DNA, amino acid (AA), peptide) through the pore. Here a method based on reversing the pore voltage and changing the solution pH is described. A simplified Fokker-Planck model shows mean translocation times of 1-10 ms in a nanopore of length 10 nm. Simulations show that a positive-negative voltage profile can trap an analyte for [~]1 ms. This method can be used for free nucleotides, single AAs, oligonucleotides, and oligopeptides. Its applicability to existing nanopore sensing and sequencing techniques and implementation issues are discussed.

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Sampath, G.. 2021-01-13. A counterintuitive method for slowing down an analyte in a nanopore and its application to polymer sequencing. https://doi.org/10.1101/2021.01.11.426231

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