GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

Norman, A. R.; Byrnes, L.; Reiter, J. R.

doi:10.1101/2021.01.07.425754

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bioRxiv · 10.1101/2021.01.07.425754

GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

Norman, A. R.·Byrnes, L.·Reiter, J. R.

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Abstract

GC-1 spg is an immortalized cell line derived from an adult mouse testis and reported to be most similar to spermatocytes, a male germ cell-type. However, immunofluorescence indicates that GC-1 spg cells express WT1, a marker of testis somatic cells, and do not express markers of germ cells. Transcriptomic profiling indicate GC-1 cells are most similar to Leydig cells. Therefore, we conclude that GC-1 spg cells are most similar to testis somatic cells.

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Norman, A. R., Byrnes, L., Reiter, J. R.. 2021-01-07. GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells. https://doi.org/10.1101/2021.01.07.425754

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