Antibody Humanization: Intra and Inter VH-VL Non-bond Energy Sort Best Humanized Antibody

Zhong, Q.

doi:10.1101/2021.01.06.425645

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bioRxiv · 10.1101/2021.01.06.425645

Antibody Humanization: Intra and Inter VH-VL Non-bond Energy Sort Best Humanized Antibody

Zhong, Q.

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Abstract

Humanization of non-human derived antibody is necessary to reduce the immunogenicity of antibody drug. In recent years, computer aided antibody humanization became an efficient and rapid routine process. We report here a new computational humanization pipeline which includes CDR grafting onto the crystal structures of homologous antibody and CDR grafted humanized antibody structures. Then, the intra and inter VH-VL binding energies are calculated and sorted for optimized humanized antibody. The resulted humanized antibodies are ranked and compared to experimental dataset, which confirms the validity of humanized antibody design by intra and inter VH-VL binding energy.

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Zhong, Q.. 2021-01-08. Antibody Humanization: Intra and Inter VH-VL Non-bond Energy Sort Best Humanized Antibody. https://doi.org/10.1101/2021.01.06.425645

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