Optogenetic activation of Gq signaling in astrocytes yields stimulation-specific effects on basal hippocampal synaptic excitation and inhibition

Courtney, C. D.; Sobieski, C.; Ramakrishnan, C.; Ingram, R. J.; Wojnowski, N. M.; DeFazio, R. A.; Deisseroth, K.; Christian-Hinman, C. A.

doi:10.1101/2021.01.06.425606

bioRxiv · 10.1101/2021.01.06.425606

Optogenetic activation of Gq signaling in astrocytes yields stimulation-specific effects on basal hippocampal synaptic excitation and inhibition

Courtney, C. D.·Sobieski, C.·Ramakrishnan, C.·Ingram, R. J.·Wojnowski, N. M.·DeFazio, R. A.·Deisseroth, K.·Christian-Hinman, C. A.

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Abstract

Astrocytes play active roles at synapses and can monitor, respond, and adapt to local synaptic activity. To investigate this relationship, more tools that can selectively activate native G protein signaling pathways in astrocytes with both spatial and temporal precision are needed. Here, we tested AAV8-GFAP-Opto1AR-eYFP (Opto1AR), a viral vector to enable activation of Gq signaling in astrocytes via light-sensitive 1-adrenergic receptors. To determine if stimulating astrocytic Opto1AR modulates hippocampal synaptic transmission, recordings were made in CA1 pyramidal cells with surrounding astrocytes expressing Opto1AR, channelrhodopsin (ChR2), or GFP. Both high-frequency (20 Hz, 45-ms light pulses, 5 mW, 5 min) and low-frequency (0.5 Hz, 1-s pulses at increasing 1, 5, and 10 mW intensities, 90 s per intensity) blue light stimulation were tested. 20 Hz Opto1AR stimulation increased both inhibitory and excitatory postsynaptic current (IPSC and EPSC) frequency, and the mIPSC effect was largely reversible within 20 min. By contrast, low-frequency stimulation of Opto1AR did not modulate either IPSCs or EPSCs, whereas the same stimulation of astrocytic ChR2 was effective. These data demonstrate that Opto1AR activation in astrocytes changes synaptic excitation and inhibition in a stimulation-sensitive manner, demonstrating the efficacy and utility of GFAP-Opto1AR as a tool in studying astrocyte-neuron interactions.

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Courtney, C. D., Sobieski, C., Ramakrishnan, C., Ingram, R. J., Wojnowski, N. M., DeFazio, R. A., Deisseroth, K., Christian-Hinman, C. A.. 2021-01-08. Optogenetic activation of Gq signaling in astrocytes yields stimulation-specific effects on basal hippocampal synaptic excitation and inhibition. https://doi.org/10.1101/2021.01.06.425606

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