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bioRxiv · 10.1101/2020.12.31.424987

Paired heavy and light chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses

Abstract

Understanding protective mechanisms of antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We discovered a new antibody, 910-30, that targets the SARS-CoV-2 ACE2 receptor binding site as a member of a public antibody response encoded by IGHV3-53/IGHV3-66 genes. We performed sequence and structural analyses to explore how antibody features correlate with SARS-CoV-2 neutralization. Cryo-EM structures of 910-30 bound to the SARS-CoV-2 spike trimer revealed its binding interactions and ability to disassemble spike. Despite heavy chain sequence similarity, biophysical analyses of IGHV3-53/3-66 antibodies highlighted the importance of native heavy:light pairings for ACE2 binding competition and for SARS-CoV-2 neutralization. We defined paired heavy:light sequence signatures and determined antibody precursor prevalence to be ~1 in 44,000 human B cells, consistent with public antibody identification in several convalescent COVID-19 patients. These data reveal key structural and functional neutralization features in the IGHV3-53/3-66 public antibody class to accelerate antibody-based medical interventions against SARS-CoV-2. HighlightsO_LIA molecular study of IGHV3-53/3-66 public antibody responses reveals critical heavy and light chain features for potent neutralization C_LIO_LICryo-EM analyses detail the structure of a novel public antibody class member, antibody 910-30, in complex with SARS-CoV-2 spike trimer C_LIO_LICryo-EM data reveal that 910-30 can both bind assembled trimer and can disassemble the SARS-CoV-2 spike C_LIO_LISequence-structure-function signatures defined for IGHV3-53/3-66 class antibodies including both heavy and light chains C_LIO_LIIGHV3-53/3-66 class precursors have a prevalence of 1:44,000 B cells in healthy human antibody repertoires C_LI

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BibTeXRIS

Banach, B. B., Cerutti, G., Fahad, A. S., Shen, C.-H., Oliveira de Souza, M., Katsamba, P. S., Tsybovsky, Y., Wang, P., Nair, M. S., Huang, Y., Francino Urdaniz, I. M., Steiner, P. J., Gutierrez-Gonzalez, M., Liu, L., Lopez Acevedo, S. N., Nazzari, A., Wolfe, J. R., Luo, Y., Olia, A. S., Teng, I.-T., Yu, J., Zhou, T., Reddem, E. R., Bimela, J., Pan, X., Madan, B., Laflin, A. D., Nimrania, R., Yuen, K.-T., Whitehead, T. A., Ho, D. D., Kwong, P. D., Shapiro, L., DeKosky, B. J.. 2021-01-03. Paired heavy and light chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses. https://doi.org/10.1101/2020.12.31.424987

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