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bioRxiv · 10.1101/2020.12.23.424157

Prenatal THC does not affect female mesolimbic dopaminergic system in preadolescent rats

Traccis, F.·Serra, V.·Sagheddu, C.·Congiu, M.·Saba, P.·Giua, G.·Devoto, P.·Frau, R.·Cheer, J. F.·Melis, M.
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Abstract

Cannabis use among pregnant women is increasing worldwide along with permissive sociocultural attitudes towards it. Prenatal cannabis exposure (PCE), however, is associated with adverse outcome among offspring ranging from reduced birth weight to child psychopathology. We have previously shown that male rat offspring prenatally exposed to {Delta}9-tetrahydrocannabinol (THC), a rat model of PCE, exhibit extensive molecular, cellular and synaptic changes in dopamine neurons of the ventral tegmental area (VTA), resulting in a susceptible mesolimbic dopamine system associated with a psychotic-like endophenotype. This phenotype only reveals itself upon a single exposure to THC in males but not females. Here, we characterized the impact of PCE on female behaviors and mesolimbic dopamine system function by combining in vivo single-unit extracellular recordings in anesthetized animals and ex vivo patch clamp recordings, along with neurochemical and behavioral analyses. We find that PCE female offspring do not show any spontaneous or THC-induced behavioral disease-relevant phenotypes. The THC-induced increase of dopamine levels in nucleus accumbens was reduced in PCE female offspring, even when VTA dopamine activity in vivo and ex vivo did not differ compared to control. These findings indicate that PCE impacts mesolimbic dopamine function and its related-behavioral domains in a sex-dependent manner and warrant further investigations to decipher the mechanisms determining this sex-related protective effect from intrauterine THC exposure. HighlightsO_LIPCE female offspring do not manifest a disease-relevant phenotype C_LIO_LIPrenatal THC does not affect female dopaminergic neurons C_LIO_LIPCE female mesolimbic dopamine function is less responsive to acute THC C_LI

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Traccis, F., Serra, V., Sagheddu, C., Congiu, M., Saba, P., Giua, G., Devoto, P., Frau, R., Cheer, J. F., Melis, M.. 2020-12-24. Prenatal THC does not affect female mesolimbic dopaminergic system in preadolescent rats. https://doi.org/10.1101/2020.12.23.424157

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