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bioRxiv · 10.1101/2020.12.23.423990

Evolution of a virus-like architecture and packaging mechanism in arepurposed bacterial protein

Tetter, S.·Terasaka, N.·Steinauer, A.·Bingham, R. J.·Clark, S.·Scott, A. P.·Patel, N.·Leibundgut, M.·Wroblewski, E.·Ban, N.·Stockley, P. G.·Twarock, R.·Hilvert, D.
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Abstract

Viruses are ubiquitous pathogens of global impact. Prompted by the hypothesis that their earliest progenitors recruited host proteins for virion formation, we have used stringent laboratory evolution to convert a bacterial enzyme lacking affinity for nucleic acids into an artificial nucleocapsid that efficiently packages and protects multiple copies of its own encoding mRNA. Revealing remarkable convergence on the molecular hallmarks of natural viruses, the accompanying changes reorganized the protein building blocks into an interlaced 240-subunit icosahedral capsid impermeable to nucleases, while emergence of a robust RNA stem-loop packaging cassette ensured high encapsidation yields and specificity. In addition to evincing a plausible evolutionary pathway for primordial viruses, these findings highlight practical strategies for developing non-viral carriers for diverse vaccine and delivery applications.

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Tetter, S., Terasaka, N., Steinauer, A., Bingham, R. J., Clark, S., Scott, A. P., Patel, N., Leibundgut, M., Wroblewski, E., Ban, N., Stockley, P. G., Twarock, R., Hilvert, D.. 2020-12-23. Evolution of a virus-like architecture and packaging mechanism in arepurposed bacterial protein. https://doi.org/10.1101/2020.12.23.423990

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