Isothermal Amplification of Nucleic Acids with Ladder-shape Melting Curve

Wang, D.; Wang, Y.; Zhang, M.; Zhang, Y.; Sun, J.; Song, C.; Xiao, F.; Ping, Y.; Chen, P.; Hu, Y.; Wang, C.; Liu, Y.

doi:10.1101/2020.12.17.423190

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bioRxiv · 10.1101/2020.12.17.423190

Isothermal Amplification of Nucleic Acids with Ladder-shape Melting Curve

Wang, D.·Wang, Y.·Zhang, M.·Zhang, Y.·Sun, J.·Song, C.·Xiao, F.·Ping, Y.·Chen, P.·Hu, Y.·Wang, C.·Liu, Y.

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Abstract

A novel method termed isothermal amplification of nucleic acids with ladder-shape melting curve (LMCP) was developed in the study. In this method, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase (large fragment) were employed to amplify the internal transcribed spacer (ITS) of Oryza sativa with ladder-shape melting curve. Our results demonstrated that the LMCP assay with nested primers was 50-fold more sensitive and one-hour faster than the LAMP assay with the same level of specificity. The LMCP method has the potential to be used for the prevention and control of the emerging epidemics.

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Wang, D., Wang, Y., Zhang, M., Zhang, Y., Sun, J., Song, C., Xiao, F., Ping, Y., Chen, P., Hu, Y., Wang, C., Liu, Y.. 2020-12-18. Isothermal Amplification of Nucleic Acids with Ladder-shape Melting Curve. https://doi.org/10.1101/2020.12.17.423190

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