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bioRxiv · 10.1101/2020.12.17.421636

RAD51AP1 mediates RAD51 activity through nucleosome interaction

Pires, E.·Sharma, N.·Selemenakis, P.·Wu, B.·Huang, Y.·Zhao, W.·Wiese, C.
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Abstract

RAD51 Associated Protein 1 (RAD51AP1) is a key protein in the homologous recombination DNA repair pathway (HR). Loss of RAD51AP1 leads to defective HR, genome instability and telomere erosion. RAD51AP1 physically interacts with the RAD51 recombinase and promotes RAD51-mediated capture of the donor DNA, synaptic complex assembly and displacement-loop formation when tested with synthetic, nucleosome-free DNA substrates in vitro. In cells, however, DNA is packaged into chromatin, posing an additional barrier to the complexities of the HR reaction. How RAD51AP1 functions as an HR activator in the context of chromatin has remained unclear. In this study, we show that RAD51AP1 binds to Nucleosome Core Particles (NCPs). We identified a C-terminal region in RAD51AP1 and its previously mapped DNA binding domain as critical for mediating the association between RAD51AP1 and both the NCP and the histone octamer. We show that RAD51AP1 is capable of promoting duplex DNA capture and initiating joint-molecule formation with the NCP and chromatinized template DNA, respectively. Together, our results suggest that RAD51AP1 directly assists the RAD51-mediated search of donor DNA in chromatin. We present a model, in which RAD51AP1 anchors the DNA template through affinity for its nucleosomes to the RAD51-ssDNA nucleoprotein filament.

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Pires, E., Sharma, N., Selemenakis, P., Wu, B., Huang, Y., Zhao, W., Wiese, C.. 2020-12-17. RAD51AP1 mediates RAD51 activity through nucleosome interaction. https://doi.org/10.1101/2020.12.17.421636

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