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bioRxiv · 10.1101/2020.12.16.422888

MAPPER: A new image analysis pipeline unmasks differential regulation of Drosophila wing features

Abstract

Phenomics requires quantification of large volumes of image data, necessitating high throughput image processing approaches. Existing image processing pipelines for Drosophila wings, a powerful model for studying morphogenesis, are limited in speed, versatility, and precision. To overcome these limitations, we developed MAPPER, a fully-automated machine learning-based pipeline that quantifies high dimensional phenotypic signatures, with each dimension representing a unique morphological feature. MAPPER magnifies the power of Drosophila genetics by rapidly identifying subtle phenotypic differences in sample populations. To demonstrate its widespread utility, we used MAPPER to reveal new insights connecting patterning and growth across Drosophila genotypes and species. The morphological features extracted using MAPPER identified the presence of a uniform scaling of proximal-distal axis length across four different species of Drosophila. Observation of morphological features extracted by MAPPER from Drosophila wings by modulating insulin signaling pathway activity revealed the presence of a scaling gradient across the anterior-posterior axis. Additionally, batch processing of samples with MAPPER revealed a key function for the mechanosensitive calcium channel, Piezo, in regulating bilateral symmetry and robust organ growth. MAPPER is an open source tool for rapid analysis of large volumes of imaging data. Overall, MAPPER provides new capabilities to rigorously and systematically identify genotype-to-phenotype relationships in an automated, high throughput fashion. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=102 SRC="FIGDIR/small/422888v1_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@bdbecdorg.highwire.dtl.DTLVardef@139a7fdorg.highwire.dtl.DTLVardef@43d5b9org.highwire.dtl.DTLVardef@1846af9_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Kumar, N., Huizar, F., Robinett, T., Farfan-Pira, K. J., Soundarrajan, D. J., Unger, M., Brodskiy, P., Nahmad, M., Zartman, J. J.. 2020-12-18. MAPPER: A new image analysis pipeline unmasks differential regulation of Drosophila wing features. https://doi.org/10.1101/2020.12.16.422888

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