Highly multiplexed imaging of biosensors in live cells

Yang, J.-M.; Chi, W.-Y.; Liang, J.; Iglesias, P. A.; Huang, C.-H.

doi:10.1101/2020.12.11.419655

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bioRxiv · 10.1101/2020.12.11.419655

Highly multiplexed imaging of biosensors in live cells

Yang, J.-M.·Chi, W.-Y.·Liang, J.·Iglesias, P. A.·Huang, C.-H.

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Abstract

Fluorescent biosensors allow for real-time monitoring of biochemical activities in cells, but their multiplexing capacity is severely limited by the availability of spectral space. We overcome this problem by developing a set of barcoding proteins that are spectrally separable from commonly used FRET (fluorescence resonance energy transfer)-based and single-fluorophore biosensors. Mixed populations of barcoded cells expressing different biosensors can be concurrently imaged and computationally unmixed to achieve highly multiplexed tracking of biochemical activities in live cells.

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Yang, J.-M., Chi, W.-Y., Liang, J., Iglesias, P. A., Huang, C.-H.. 2020-12-11. Highly multiplexed imaging of biosensors in live cells. https://doi.org/10.1101/2020.12.11.419655

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