Data-informed discovery of hydrolytic nanozymes

Li, S.; Zhou, Z.; Tie, Z.; Wang, B.; Ye, M.; Du, L.; Cui, R.; Liu, W.; Wan, C.; Liu, Q.; Zhao, S.; Wang, Q.; Zhang, Y.; Zhang, S.; Zhang, H.; Du, Y.; Wei, H.

doi:10.1101/2020.12.08.416305

bioRxiv · 10.1101/2020.12.08.416305

Data-informed discovery of hydrolytic nanozymes

Li, S.·Zhou, Z.·Tie, Z.·Wang, B.·Ye, M.·Du, L.·Cui, R.·Liu, W.·Wan, C.·Liu, Q.·Zhao, S.·Wang, Q.·Zhang, Y.·Zhang, S.·Zhang, H.·Du, Y.·Wei, H.

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Abstract

Nanozyme is a collection of nanomaterials with enzyme-like activity but exhibits higher environmental tolerance and long-term stability than their natural counterparts. Improving the catalytic activity and expanding the category of nanozymes are prerequisites to complement or even supersede natural enzymes. Specifically, a powerful hydrolytic nanozyme is demanded to degrade the unsustainable substance which natural enzymes hardly achieve. However, the development of hydrolytic nanozymes is still hindered by diverse hydrolytic substrates and following complicated mechanisms. Here, we apply two strategies which are informed by data to screen and predict catalytic active sites of MOF (metal-organic framework) based hydrolytic nanozymes. One is to increase the intrinsic activity by finely tuned Lewis acidity of the metal clusters. The other is to adjust the volume density of the active sites by shortening the length of ligands. Finally, we construct a Ce-FMA-MOF-based hydrolytic nanozyme with robust cleavage ability towards phosphate bonds, amide bonds, glycosidic bonds whose energy ascend in order; and even their mixture, biofilms. This work provides a rational methodology to design hydrolytic nanozyme, enriches the diversity of nanozymes, and potentially sheds a light on the evolution of enzyme engineering in the future.

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Li, S., Zhou, Z., Tie, Z., Wang, B., Ye, M., Du, L., Cui, R., Liu, W., Wan, C., Liu, Q., Zhao, S., Wang, Q., Zhang, Y., Zhang, S., Zhang, H., Du, Y., Wei, H.. 2020-12-09. Data-informed discovery of hydrolytic nanozymes. https://doi.org/10.1101/2020.12.08.416305

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