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bioRxiv · 10.1101/2020.12.04.374918

De novo transcriptome assembly and functional annotation of cobia (Rachycentron canadum): molecular approach as a base to nutritional studies

Aciole Barbosa, D.·Araujo, B. C.·Branco, G. S.·Hilsdorf, A.·Nunes, L. R.·Moreira, R. G.·Menegidio, F. B.
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Abstract

Cobia (Rachycentron canadum) is a marine teleost species with great productive potential worldwide. However, the genomic information currently available for this species in public databases is limited. Such lack of information hinders gene expression assessments that might bring forward novel insights into the physiology, ecology, evolution, and genetics of this potential aquaculture species. In this study, we report the first de novo transcriptome assembly of R. canadum liver, improving the availability of novel gene sequences for this species. Illumina sequencing of liver transcripts generated 1,761,965,794 raw reads, which were filtered into 1,652,319,304 high-quality reads. De novo assembly resulted in 101,789 unigenes and 163,096 isoforms, with an average length of 950.61 and 1,617.34 nt, respectively. Moreover, we found that 126,013 of these transcripts bear potentially coding sequences, and 125,993 of these elements (77.3%) correspond to functionally annotated genes found in six different databases. We also identified 701 putative ncRNA and 35,414 putative lncRNA. Interestingly, homologues for 410 of these putative lncRNAs have already been observed in previous analyzes with Danio rerio, Lates calcarifer, Seriola lalandi dorsalis, Seriola dumerili or Echeneis naucrates. Finally, we identified 7,894 microsatellites related to cobias putative lncRNAs. Thus, the information derived from the transcriptome assembly described herein will likely assist future nutrigenomics and breeding programs involving this important fish farming species.

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Aciole Barbosa, D., Araujo, B. C., Branco, G. S., Hilsdorf, A., Nunes, L. R., Moreira, R. G., Menegidio, F. B.. 2020-12-06. De novo transcriptome assembly and functional annotation of cobia (Rachycentron canadum): molecular approach as a base to nutritional studies. https://doi.org/10.1101/2020.12.04.374918

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