A Genetic Screen for Temperature-sensitive Morphogenesis-defective Caenorhabditis elegans Mutants

Abstract

Morphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of [~]200 such Caenorhabditis elegans mutants, we have identified 17 that have highly penetrant embryonic morphogenesis defects after upshifts from the permissive to the restrictive temperature, just prior to the cell shape changes that mediate elongation of the ovoid embryo into a vermiform larva. Using whole genome sequencing, we identified the causal mutations in seven affected genes. These include three genes that have roles in producing the extracellular matrix, which is known to affect the morphogenesis of epithelial tissues in multicellular organisms. The rib-1 and rib-2 genes encode glycosyltransferases, and the emb-9 gene encodes a collagen subunit. We also used live imaging to characterize epidermal cell shape dynamics in one mutant, or1219ts, and observed cell elongation defects during dorsal intercalation and ventral enclosure that may be responsible for the body elongation defects. These results indicate that our screen has identified factors that influence morphogenesis and provides a platform for advancing our understanding of this fundamental biological process. SUMMARYWe performed a systematic, forward genetics screen for temperature-sensitive embryonic-lethal (TS-EL) Caenorhabditis elegans mutants that are specifically defective in embryonic morphogenesis. By taking advantage of temperature-upshifts, we identified several essential genes influencing morphogenesis. We also demonstrated that one mutant has defects in epidermal cell shape changes that likely account for the failure in morphogenesis. The TS-EL mutants we identified will be useful tools for advancing our understanding of the gene networks controlling cell shape changes and movements during morphogenesis.