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bioRxiv · 10.1101/2020.12.01.403949

Myotube hypertrophy is associated with cancer-like metabolic reprogramming and limited by PHGDH

Abstract

Muscle fiber size and oxidative metabolism are inversely related, suggesting that a glycolytic metabolism may offer a growth advantage in muscle fibers. However, the mechanisms underlying this advantage remains unknown. Nearly 100 years ago, Warburg reported that cancer cells take up more glucose to produce glycolytic intermediates for anabolic reactions such as amino acid-protein synthesis. The aim of this study was to test whether glycolysis contributes to anabolic signalling responses and hypertrophy in post-mitotic muscle cells. Skeletal muscle hypertrophy was induced in vitro by treating mouse C2C12 myotubes with IGF-1. 14C glucose was added to differentiation medium and radioactivity in isolated protein was measured. We exposed differentiated C2C12 and primary mouse myotubes, to 2-deoxyglucose (2DG) and PHGDH siRNA upon which we assessed myotube diameter and signaling pathways involved in the regulation of muscle fiber size. Here, we present evidence that, hypertrophying C2C12 myotubes undergo a cancer-like metabolic reprogramming. First, IGF-1-induced C2C12 myotube hypertrophy increases shunting of carbon from glucose into protein. Second, reduction of glycolysis through 2-deoxy-D-glucose (2DG) lowers C2C12 and primary myotube size 16-40%. Third, reducing the cancer metabolism-associated enzyme PHGDH decreases C2C12 and primary myotube size 25-52%, whereas PHGDH overexpression increases C2C12 myotube size {approx}20%. Fourth, the muscle hypertrophy-promoting kinase AKT regulates PHGDH expression. Together these results suggest that glycolysis is important for hypertrophying C2C12 myotubes by reprograming their metabolism similar to cancer cells.

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BibTeXRIS

Stadhouders, L. E., Verbrugge, S. A., Smith, J. A., Gabriel, B. M., Hammersen, T. D., Kolijn, D., Vogel, I. S., Mohamed, A. M., De Wit, G. M., Offringa, C., Hoogaars, W. M., Gehlert, S., Wackerhage, H., Jaspers, R. T.. 2020-12-01. Myotube hypertrophy is associated with cancer-like metabolic reprogramming and limited by PHGDH. https://doi.org/10.1101/2020.12.01.403949

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