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bioRxiv · 10.1101/2020.11.30.403311

Freeze-frame imaging of dendritic calcium signals with TubuTag

Perez-Alvarez, A.·Huhn, F.·Duerst, C. D.·Franzelin, A.·Lamothe-Molina, P.·Oertner, T. G.
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Abstract

The extensive dendritic arbor of neurons is thought to be actively involved in the processing of information. Dendrites contain a rich diversity of ligand- and voltage-activated ion channels as well as metabotropic receptors. In addition, they are capable of releasing calcium from intracellular stores. Under specific conditions, large neurons produce calcium spikes that are locally restricted to a dendritic section. To investigate calcium signaling in dendrites, we introduce TubuTag, a genetically encoded calcium sensor anchored to the cytoskeleton. TubuTag integrates cytoplasmic calcium signals by irreversible photoconversion from green to red fluorescence when illuminated with violet light. To image the mm-long dendritic tree of pyramidal neurons at subcellular resolution, we used a custom two-photon microscope with a large field of view. As the read-out of fluorescence can be performed several hours after photoconversion, TubuTag will help investigating dendritic signal integration and calcium homeostasis in large populations of neurons.

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Perez-Alvarez, A., Huhn, F., Duerst, C. D., Franzelin, A., Lamothe-Molina, P., Oertner, T. G.. 2020-12-02. Freeze-frame imaging of dendritic calcium signals with TubuTag. https://doi.org/10.1101/2020.11.30.403311

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