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bioRxiv · 10.1101/2020.11.10.376590

EGCG-modified bone graft to modulate the recruitment of M1 macrophage and alleviate the forming of fibrous capsule

Yao, D.·Rung, S.·CHU, C.·MAN, Y.·QU, Y.
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Abstract

In response to current trends in the modification of guided bone regeneration (GBR) materials, we aimed to build upon our previous studies on epigallocatechin-3-gallate (EGCG) by immersing a commonly used bone graft primarily composed of hydroxyapatite (HA) in EGCG solution, expecting to obtain superior bone-material integration after implantation. Bone grafts are commonly used for bone repair, in which the bone extracellular matrix is stimulated to promote osteogenesis. However, due to its pro-fibrosis effect, this osteoconductive material commonly exhibits implant failure. In addition to providing a basic release profile of EGCG-modified bone graft (E-HA) to clarify the relationship between this material and the environment, we have examined the integration effect via subcutaneous implantation experiments. In this manner, we have assessed the aggregation of pro-inflammatory macrophages, initial angiogenesis, the formation of fibrous capsules, and an enhanced cell viability observed in cultured RAW 264.7 cells. Among these results, we focus on pro-inflammatory macrophages due to their close relationship with fibrosis, which is the most important process in the immune response. Immunofluorescent staining results showed that E-HA substantially compromised the formation of fibrous capsules in hematoxylin-eosin-stained sections, which exhibited less pro-inflammatory macrophage recruitment; meanwhile, the cell viability and primary angiogenesis were improved. This work lays the foundation for future studies on GBR.

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Yao, D., Rung, S., CHU, C., MAN, Y., QU, Y.. 2020-11-10. EGCG-modified bone graft to modulate the recruitment of M1 macrophage and alleviate the forming of fibrous capsule. https://doi.org/10.1101/2020.11.10.376590

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