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bioRxiv · 10.1101/2020.11.03.367177

Intracellular sterol sensing controls intestinal B cell differentiation

Trindade, B. C.·Ceglia, S.·Berthelette, A.·Raso, F.·Howley, K.·Muppidi, J. R.·Reboldi, A.
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Abstract

Intestinal B cell responses are critical for the maintenance of gut homeostasis, yet environmental signals that control B cell metabolism and effector function remain poorly characterized. Here, we show that Peyers patches germinal center (GC) B cells are sensitive to 25-hydroxycholesterol (25-HC), an oxidized metabolite of cholesterol produced by the enzyme cholesterol 25-hydroxylase (CH25H). In mice lacking CH25H, antigen-specific GC B cells show an increased cholesterol metabolic signature and preferentially differentiate in plasma cells (PCs), thereby inducing a stronger intestinal IgA response upon immunization or infection. GC B cells express the sterol sensor SREBP2 and use it to sense 25-HC. Deletion of SREBP2 from GC B cells prevents PC differentiation and forces the maintenance of GC identity. GC localized oxysterol production by follicular dendritic cells is central in dictating GC metabolism and imposing B cell fate. Our findings show that the 25-HC-SREBP2 axis shapes B cell effector function in intestinal lymphoid organs and indicate that dietary cholesterol can instruct local B cell response.

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Trindade, B. C., Ceglia, S., Berthelette, A., Raso, F., Howley, K., Muppidi, J. R., Reboldi, A.. 2020-11-05. Intracellular sterol sensing controls intestinal B cell differentiation. https://doi.org/10.1101/2020.11.03.367177

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