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bioRxiv · 10.1101/2020.10.18.344432

Programmable Aggregation of Artificial Cells with DNA Signals

Qiu, H.·Li, F.·Du, Y.·Li, R.·Hyun, J. Y.·Lee, S. Y.·Choi, J. H.
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Abstract

Cell aggregation is a complex behavior, which is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as a signaling molecule. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme, and that the small vesicles dissociate when receiving release signals. This work provides new possibilities for building synthetic protocells capable of chemical communication and coordination.

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Qiu, H., Li, F., Du, Y., Li, R., Hyun, J. Y., Lee, S. Y., Choi, J. H.. 2020-10-18. Programmable Aggregation of Artificial Cells with DNA Signals. https://doi.org/10.1101/2020.10.18.344432

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