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bioRxiv · 10.1101/2020.10.15.341743

Antibody Response to a Nucleocapsid Epitope as a Marker for COVID-19 Disease Severity

Abstract

Effective methods for predicting COVID-19 disease trajectories are urgently needed. Here, ELISA and coronavirus antigen microarray (COVAM) analysis mapped antibody epitopes in the plasma of COVID-19 patients (n = 86) experiencing a wide-range of disease states. The experiments identified antibodies to a 21-residue epitope from nucleocapsid (termed Ep9) associated with severe disease, including admission to the ICU, requirement for ventilators, or death. Importantly, anti-Ep9 antibodies can be detected within six days post-symptom onset and sometimes within one day. Furthermore, anti-Ep9 antibodies correlate with various comorbidities and hallmarks of immune hyperactivity. We introduce a simple-to-calculate, disease risk factor score to quantitate each patients comorbidities and age. For patients with anti-Ep9 antibodies, scores above 3.0 predict more severe disease outcomes with a 13.42 Likelihood Ratio (96.7% specificity). The results lay the groundwork for a new type of COVID-19 prognostic to allow early identification and triage of high-risk patients. Such information could guide more effective therapeutic intervention. Significance statementThe COVID-19 pandemic has resulted in over two million deaths worldwide. Despite efforts to fight the virus, the disease continues to overwhelm hospitals with severely ill patients. Diagnosis of COVID-19 is readily accomplished through a multitude of reliable testing platforms; however, prognostic prediction remains elusive. To this end, we identified a short epitope from the SARS-CoV-2 nucleocapsid protein and also a disease risk factor score based upon comorbidities and age. The presence of antibodies specifically binding to this epitope plus a score cutoff can predict severe COVID-19 outcomes with 96.7% specificity.

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BibTeXRIS

Sen, S., Sanders, E. C., Gabriel, K. N., Miller, B. M., Isoda, H. M., Salcedo, G. S., Garrido, J. E., Dyer, R. P., Nakajima, R., Jain, A., Santos, A. M., Bhuvan, K., Tifrea, D. F., Ricks-Oddie, J. L., Felgner, P. L., Edwards, R. A., Majumdar, S., Weiss, G. A.. 2020-10-16. Antibody Response to a Nucleocapsid Epitope as a Marker for COVID-19 Disease Severity. https://doi.org/10.1101/2020.10.15.341743

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