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bioRxiv · 10.1101/2020.10.15.340919

Preventing neutrophil from oxygen exposure allows their basal state maintenance

Injarabian, L.·Giai Gianetto, Q.·Witko-Sarsat, V.·Marteyn, B. S.
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Abstract

Neutrophils are the most abundant circulating white blood cells and are central players of the innate immune response. During their lifecycle, neutrophils mainly evolve under low oxygen conditions (0.1 - 4% O2) to which they are well adapted. Neutrophils are atypical cells since they are mainly glycolytic, and highly susceptible to oxygen-exposure, which induces their activation and death, through mechanisms which remain currently elusive. Nevertheless, nearly all studies conducted on neutrophils are carried out under atmospheric oxygen (21%), corresponding to hyperoxic conditions. Here we investigated the impact of hyperoxia during neutrophil purification and culture on neutrophil viability, activation and cytosolic protein content. Neutrophil hyper-activation (CD62L shedding) is induced during culture under hyperoxic conditions (24h), compared to neutrophils cultured under anoxic conditions. In addition, we show that maintaining neutrophils in autologous plasma is the most suitable strategy to maintain their basal state. Our results show that manipulating neutrophils under hyperoxic conditions leads to the loss of ~100 cytosolic proteins during purification, while it does not lead to an immediate impact on neutrophils activation (CD11bhigh, CD54high, CD62Llow) or viability (DAPI+). We identified two clusters of proteins belonging to the cholesterol metabolism and to the complement and coagulation cascade pathways, which are highly susceptible to neutrophil oxygen-exposure during their purification. In conclusion, preserving neutrophil from oxygen-exposure during their manipulation - purification and culture- is recommended to avoid their experimental activation and for preserving a large set of cytosolic proteins from alteration.

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Injarabian, L., Giai Gianetto, Q., Witko-Sarsat, V., Marteyn, B. S.. 2020-10-15. Preventing neutrophil from oxygen exposure allows their basal state maintenance. https://doi.org/10.1101/2020.10.15.340919

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