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bioRxiv · 10.1101/2020.10.07.330068

NSP 11 of SARS-CoV-2 is an Intrinsically Disordered Protein

Gadhave, K.·Kumar, P.·Kumar, A.·Bhardwaj, T.·Garg, N.·Giri, R.
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Abstract

The intrinsically disordered proteins/regions (IDPs/IDPRs) are known to be responsible for multiple cellular processes and are associated with many chronic diseases. In viruses, the existence of a disordered proteome is also proven and is related to its conformational dynamics inside the host. The SARS-CoV-2 has a large proteome, in which, structure and functions of many proteins are not known yet, along with nsp11. In this study, we have performed extensive experimentation on nsp11. Our results based on the CD spectroscopy gives characteristic disordered spectrum for IDPs. Further, we investigated the conformational behavior of nsp11 in the presence of membrane mimetic environment, -helix inducer, and natural osmolyte. In the presence of negatively charged and neutral liposomes, nsp11 remains disordered. However, with SDS micelle, it adopted an -helical conformation, suggesting the helical propensity of nsp11. Finally, we again confirmed the IDP behavior of nsp11 using MD simulations. In future, this conformational dynamic study could help to clarify its functional importance in SARS-CoV-2 infection.

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Gadhave, K., Kumar, P., Kumar, A., Bhardwaj, T., Garg, N., Giri, R.. 2020-10-07. NSP 11 of SARS-CoV-2 is an Intrinsically Disordered Protein. https://doi.org/10.1101/2020.10.07.330068

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