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bioRxiv · 10.1101/2020.10.01.322974

Perilipin2 down-regulation in beta cells impairs insulin secretion under nutritional stress and damages mitochondria

Abstract

Perilipin 2 (PLIN2) is the lipid droplet (LD) protein in {beta} cells that increases under nutritional stress. Down-regulation of PLIN2 is often sufficient to reduce LD accumulation. To determine whether PLIN2 positively or negatively affects {beta} cell function under nutritional stress, PLIN2 was down-regulated in mouse {beta} cells, INS1 cells, and human islet cells. {beta} cell specific deletion of PLIN2 in mice on a high fat diet reduced glucose-stimulated insulin secretion (GSIS) in vivo and in vitro. Down-regulation of PLIN2 in INS1 cells blunted GSIS after 24 h incubation with 0.2 mM palmitic acids. Down-regulation of PLIN2 in human pseudoislets cultured at 5.6 mM glucose impaired both phases of GSIS, indicating that PLIN2 is critical for GSIS. Down-regulation of PLIN2 decreased specific OXPHOS proteins in all three models and reduced oxygen consumption rates in INS1 cells and mouse islets. Moreover, we found that PLIN2 deficient INS1 cells increased the distribution of a fluorescent oleic acid analog to mitochondria and showed signs of mitochondrial stress as indicated by susceptibility to fragmentation and alterations of acyl-carnitines and glucose metabolites. Collectively, PLIN2 in {beta} cells have an important role in preserving insulin secretion, {beta} cell metabolism and mitochondrial function under nutritional stress.

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BibTeXRIS

Mishra, A., Liu, S., Promes, J., Harata, M., Sivitz, W., Fink, B., Bhardwaj, G., O'Neill, B., Kang, C., Sah, R., Strack, S., Stephens, S., King, T., Jackson, L., Greenberg, A., Anokye-Danso, F., Ahima, R., Ankrum, J. A., Imai, Y.. 2020-10-01. Perilipin2 down-regulation in beta cells impairs insulin secretion under nutritional stress and damages mitochondria. https://doi.org/10.1101/2020.10.01.322974

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