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bioRxiv · 10.1101/2020.09.28.317461

A deep learning algorithm to translate and classify cardiac electrophysiology: From iPSC-CMs to adult cardiac cells

Aghasafari, P.·Yang, P.-C.·Kernik, D. C.·Sakamoto, K.·Kanda, Y.·Kurokawa, J.·Vorobyov, I.·Clancy, C. E.
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Abstract

The development of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) has been a critical in vitro advance in the study of patient-specific physiology, pathophysiology and pharmacology. We designed a new deep learning multitask network approach intended to address the low throughput, high variability and immature phenotype of the iPSC-CM platform. It was trained using simulated action potential (AP) data and applied to classify cells into the drug-free and drugged categories and to predict the impact of electrophysiological perturbation across the continuum of aging from the immature iPSC-CMs to the adult ventricular myocytes. The phase of the AP extremely sensitive to perturbation due to a steep rise of the membrane resistance was found to contain the key information required for successful network multitasking. We also demonstrated successful translation of both experimental and simulated iPSC-CM AP data validating our network by prediction of experimental drug-induced effects on adult cardiomyocyte APs by the latter.

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Aghasafari, P., Yang, P.-C., Kernik, D. C., Sakamoto, K., Kanda, Y., Kurokawa, J., Vorobyov, I., Clancy, C. E.. 2020-09-29. A deep learning algorithm to translate and classify cardiac electrophysiology: From iPSC-CMs to adult cardiac cells. https://doi.org/10.1101/2020.09.28.317461

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