High-throughput whole-brain mapping of rhesus monkey at micron resolution

Xu, F.; Shen, Y.; Ding, L.; Yang, C.-Y.; Tan, H.; Wang, H.; Zhu, Q.; Xu, R.; Wu, F.; Xu, C.; Li, Q.; Su, P.; Zhang, L. I.; Dong, H.; Desimone, R.; Hu, X.; Lau, P.-M.; Bi, G.-Q.

doi:10.1101/2020.09.25.313395

bioRxiv · 10.1101/2020.09.25.313395

High-throughput whole-brain mapping of rhesus monkey at micron resolution

Xu, F.·Shen, Y.·Ding, L.·Yang, C.-Y.·Tan, H.·Wang, H.·Zhu, Q.·Xu, R.·Wu, F.·Xu, C.·Li, Q.·Su, P.·Zhang, L. I.·Dong, H.·Desimone, R.·Hu, X.·Lau, P.-M.·Bi, G.-Q.

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Abstract

Whole-brain mesoscale mapping of primates has been hindered by large brain size and the relatively low throughput of available microscopy methods. Here, we present an integrative approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed, large-scale, volumetric fluorescence microscopy, capable of completing whole-brain imaging of a rhesus monkey at 1 {micro}m x 1 {micro}m x 2.5 {micro}m voxel resolution within 100 hours. A progressive strategy is developed for high-efficiency, long-range tracing of individual axonal fibers through the dataset of hundreds of terabytes, establishing a "Serial sectioning and clearing, 3-dimensional Microscopy, with semi-Automated Reconstruction and Tracing" (SMART) pipeline. This system supports effective connectome-scale mapping of large primates that reveals distinct features of thalamocortical projections of the rhesus monkey brain at the level of individual axonal fibers.

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Xu, F., Shen, Y., Ding, L., Yang, C.-Y., Tan, H., Wang, H., Zhu, Q., Xu, R., Wu, F., Xu, C., Li, Q., Su, P., Zhang, L. I., Dong, H., Desimone, R., Hu, X., Lau, P.-M., Bi, G.-Q.. 2020-09-26. High-throughput whole-brain mapping of rhesus monkey at micron resolution. https://doi.org/10.1101/2020.09.25.313395

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