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bioRxiv · 10.1101/2020.09.24.298851

ADPriboDB v2.0: An Updated Database of ADP-ribosylated Proteins

Ayyappan, V.·Wat, R.·Barber, C.·Vivelo, C.·Gauch, K.·Visanpattanasin, P.·Cook, G.·Sazeides, C.·Leung, A. K. L.
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Abstract

ADP-ribosylation is a protein modification responsible for biological processes such as DNA repair, RNA regulation, cell cycle, and biomolecular condensate formation. Dysregulation of ADP-ribosylation is implicated in cancer, neurodegeneration, and viral infection. We developed ADPriboDB (adpribodb.leunglab.org) to facilitate studies in uncovering insights into the mechanisms and biological significance of ADP-ribosylation. ADPriboDB 2.0 serves as a one-stop repository comprising 48,346 entries and 9,097 ADP-ribosylated proteins, of which 6,708 were newly identified since the original database release. In this updated version, we provide information regarding the sites of ADP-ribosylation in 32,946 entries. The wealth of information allows us to interrogate existing databases or newly available data. For example, we found that ADP-ribosylated substrates are significantly associated with the recently identified human protein interaction networks associated with SARS-CoV-2, which encodes a conserved protein domain called macrodomain that binds and removes ADP-ribosylation. In addition, we create a new interactive tool to visualize the local context of ADP-ribosylation, such as structural and functional features as well as other post-translational modifications (e.g., phosphorylation, methylation and ubiquitination). This information provides opportunities to explore the biology of ADP-ribosylation and generate new hypotheses for experimental testing.

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Ayyappan, V., Wat, R., Barber, C., Vivelo, C., Gauch, K., Visanpattanasin, P., Cook, G., Sazeides, C., Leung, A. K. L.. 2020-09-25. ADPriboDB v2.0: An Updated Database of ADP-ribosylated Proteins. https://doi.org/10.1101/2020.09.24.298851

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