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bioRxiv · 10.1101/2020.09.21.306308

Starvation-induced regulation of carbohydrate transport at the blood-brain barrier is TGF-β-signaling dependent

Hertenstein, H.·McMullen, E.·Weiler, A.·Volkenhoff, A.·Becker, H. M.·Schirmeier, S.
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Abstract

During hunger or malnutrition animals prioritize alimentation of the brain over other organs to ensure its function and thus their survival. This so-called brain sparing is described from Drosophila to humans. However, little is known about the molecular mechanisms adapting carbohydrate transport. Here, we used Drosophila genetics to unravel the mechanisms operating at the blood-brain barrier (BBB) under nutrient restriction. During starvation, expression of the carbohydrate transporter Tret1-1 is increased to provide more efficient carbohydrate uptake. Two mechanisms are responsible for this increase. Similarly to the regulation of mammalian GLUT4, Rab-dependent intracellular shuttling is needed for Tret1-1 integration into the plasma membrane, even though Tret1-1 regulation is independent of insulin signaling. In addition, starvation induces transcriptional upregulation controlled by TGF-{beta} signaling. Considering TGF-{beta}-dependent regulation of the glucose transporter GLUT1 in murine chondrocytes, our study reveals an evolutionarily conserved regulatory paradigm adapting the expression of sugar transporters at the BBB.

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Hertenstein, H., McMullen, E., Weiler, A., Volkenhoff, A., Becker, H. M., Schirmeier, S.. 2020-09-22. Starvation-induced regulation of carbohydrate transport at the blood-brain barrier is TGF-β-signaling dependent. https://doi.org/10.1101/2020.09.21.306308

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