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bioRxiv · 10.1101/2020.09.18.303248

Adaptation of a microfluidic qPCR system for enzyme kinetics studies

Rembeza, E.·Engqvist, M. K.
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Abstract

Microfluidic platforms offer a drastic increase in throughput while minimizing sample usage and hands-on time, which makes them important tools for large-scale biological studies. A range of such systems have been developed for enzyme activity studies, although their complexity largely hinders their application by a wider scientific community. Here we present adaptation of an easy-to-use commercial microfluidic qPCR system for performing enzyme kinetics studies. We demonstrate functionality of the Fluidigm Biomark HD system (the Fluidigm system) by determining kinetic properties of three oxidases in a resorufin-based fluorescent assay. The results obtained in the microfluidic system proved reproducible and comparable to the ones obtained in a standard microplate-based assay. With a wide range of easy-to-use, off-the-shelf components, the microfluidic system presents itself as a simple and customizable platform for high-throughput enzyme activity studies. Graphical abstract O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY

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Rembeza, E., Engqvist, M. K.. 2020-09-20. Adaptation of a microfluidic qPCR system for enzyme kinetics studies. https://doi.org/10.1101/2020.09.18.303248

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