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bioRxiv · 10.1101/2020.09.10.291088

CycleFlow quantifies cell-cycle heterogeneity in vivo

Jolly, A.·Fanti, A.-K.·Grässer, I.·Becker, N. B.·Höfer, T.
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Abstract

While the average cell-cycle length in a cell population can be derived from pulse-chase experiments, proliferative heterogeneity has been difficult to quantify. Here we describe CO_SCPLOWYCLEC_SCPLOWFO_SCPLOWLOWC_SCPLOW, a broadly applicable method that applies Bayesian inference to combined measurements of EdU incorporation and DNA content. CO_SCPLOWYCLEC_SCPLOWFO_SCPLOWLOWC_SCPLOW accurately quantifies the fraction of proliferating versus quiescent cells and the durations of cell-cycle phases of the proliferating cells in vitro and in vivo.

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Jolly, A., Fanti, A.-K., Grässer, I., Becker, N. B., Höfer, T.. 2020-09-11. CycleFlow quantifies cell-cycle heterogeneity in vivo. https://doi.org/10.1101/2020.09.10.291088

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