Efficient cooperation of chloroplasts and mitochondria enhances ATP and sucrose production

Voon, C. P.; Law, Y.-S.; Guan, X.; Xu, Z.; Chu, W.-T.; Zhang, R.; Sun, F.; Labs, M.; Pribil, M.; Leister, D.; Hronkova, M.; Kubasek, J.; Cui, Y.; Jiang, L.; Tsuyama, M.; Gardestrom, P.; Tikkanen, M.; Lim, B. L.

doi:10.1101/2020.09.09.289967

bioRxiv · 10.1101/2020.09.09.289967

Efficient cooperation of chloroplasts and mitochondria enhances ATP and sucrose production

Voon, C. P.·Law, Y.-S.·Guan, X.·Xu, Z.·Chu, W.-T.·Zhang, R.·Sun, F.·Labs, M.·Pribil, M.·Leister, D.·Hronkova, M.·Kubasek, J.·Cui, Y.·Jiang, L.·Tsuyama, M.·Gardestrom, P.·Tikkanen, M.·Lim, B. L.

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Abstract

Efficient photosynthesis requires a balance of ATP and NADPH production/consumption in chloroplasts and the exportation of reducing equivalents from chloroplasts is important for balancing stromal ATP/NADPH ratio. Here we showed that the overexpression of purple acid phosphatase 2 on the outer membranes of chloroplasts and mitochondria can streamline the production and consumption of reducing equivalents in these two organelles, respectively. A higher capacity of consumption of reducing equivalents in mitochondria can indirectly help chloroplasts to balance the ATP/NADPH ratio in stroma and recycle NADP+, the electron acceptors of the linear electron flow. A higher rate of ATP and NADPH production from the linear electron flow, a higher capacity of carbon fixation by the Calvin-Benson-Bassham cycle and a greater consumption of NADH in mitochondria, enhance photosynthesis in the chloroplasts, ATP production in the mitochondria, sucrose synthesis in the cytosol, and eventually boosting plant growth and seed yields in the overexpression lines.

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Voon, C. P., Law, Y.-S., Guan, X., Xu, Z., Chu, W.-T., Zhang, R., Sun, F., Labs, M., Pribil, M., Leister, D., Hronkova, M., Kubasek, J., Cui, Y., Jiang, L., Tsuyama, M., Gardestrom, P., Tikkanen, M., Lim, B. L.. 2020-09-11. Efficient cooperation of chloroplasts and mitochondria enhances ATP and sucrose production. https://doi.org/10.1101/2020.09.09.289967

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