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bioRxiv · 10.1101/2020.09.03.281097

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin stability

Abstract

TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease characterized by impaired growth and increased tumorigenesis, whose cellular etiology is poorly understood. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we characterized these centriolar protein assemblies (Cenpas) to uncover the mechanism of action of TRIM37. We established that an atypical de novo assembly pathway is notably involved in forming Cenpas, which can nevertheless trigger further centriole assembly and act as MTOCs. We found also that Cenpas are present and act similarly in Mulibrey patient cells. Through correlative light electron microscopy, we uncovered that Cenpas correspond to centriole related structures and elongated electron-dense structures with stripes. Importantly, we established that TRIM37 regulates the stability and solubility of the centriolar protein Centrobin. Our findings suggest that elongated Centrobin assemblies are a major constituent of the striped electron dense structures. Furthermore, we established that Cenpas formation upon TRIM37 depletion requires PLK4 activity, as well as two parallel pathways relying respectively on Centrobin and PLK1. Overall, our work uncovers how TRIM37 prevents the formation of Cenpas that would otherwise threaten genome integrity, including possibly in Mulibrey patients.

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BibTeXRIS

Gönczy, P., Balestra, F. R., Wolf, B., Dominguez-Calvo, A., Buff, A., Averink, T., Lipsanen-Nyman, M., Busso, C., Huertas, P., Rios, R. M.. 2020-09-04. TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin stability. https://doi.org/10.1101/2020.09.03.281097

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