Differences in Chemo-signaling Compound-Evoked Brain Activity in Male and Female Young Adults: A Pilot Study in the Role of Sexual Dimorphism in Olfactory Chemo-Signaling

Ottesen, T.; Davis, K. C.; Hobbs, L. K.; Muncy, N. M.; Stevens, N. M.; Anderson, M.; Johnson, P.; Doxey, C. R.; Richter, K.; Wang, H.; Hartley, R.; Kirwan, C. B.; Wisco, J. J.

doi:10.1101/2020.09.03.280685

bioRxiv · 10.1101/2020.09.03.280685

Differences in Chemo-signaling Compound-Evoked Brain Activity in Male and Female Young Adults: A Pilot Study in the Role of Sexual Dimorphism in Olfactory Chemo-Signaling

Ottesen, T.·Davis, K. C.·Hobbs, L. K.·Muncy, N. M.·Stevens, N. M.·Anderson, M.·Johnson, P.·Doxey, C. R.·Richter, K.·Wang, H.·Hartley, R.·Kirwan, C. B.·Wisco, J. J.

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Abstract

IntroductionPrevious studies have shown that putative pheromones 4,16-androstadien-3-one (AND) and estra-1,3,5(10),16-tetraen-3-ol (EST) cause activation in the preoptic area/anterior hypothalamus in men and women. Sex differences in neural activation patterns have been demonstrated when participants are subject to pheromone stimulation; however, whether other compounds give rise to similar neural activity has not been completely investigated. MethodsTwenty-nine young adults [16 female (21.3+/-0.54; mean yrs+/-SE), 13 male (22.85+/-0.42)] participated in a 3-block design, where participants were exposed to a scent (lavender), a synthetic male pheromone (4,16-androstadien-3b-ol; ALD), and a synthetic female pheromone (1,3,5(10),16-Estratetraen-3-ol; EST) via an automated olfactometer. Whole-brain, high-resolution (1.8mm3) functional MRI data from a Siemens Trio 3T MRI scanner were collected during all blocks. Five adults were excluded due to excessive movement. MANOVA analysis, a 2 x 3 multivariate model and analysis of 2x2 effects between sex and subsets of stimuli was done for activation over the whole brain and small volumes involved in olfaction. ResultsExploratory analysis of 2x2 effects between sex and subsets of stimuli exhibited significant interactions when assessing activations over the whole brain, and small volumes involved in olfaction. The left and right frontal poles (LFP, RFP) shows significant interaction when assessing sex with lavender and EST for whole brain analysis. For small volume analysis, the right orbitofrontal cortex (ROFC) exhibited a sex with lavender and ALD interaction, and a sex with lavender and EST interaction was observed in the left inferior frontal gyrus (LIFG). Main effects of sex, stimulus, or interaction show no differences analyzed using a 2 x 3 multivariate model. ConclusionThe study shows there is a sexually dimorphic response in the olfactory system to pheromones not previously studied. Scents like lavender do not have this same response. These distinct functional differences in activation patterns may be a result of neural development and maturation differences between sexes. Future studies should expand this pilot study and involve a younger demographic to accurately determine the age at which the olfactory response differentiates between males and females.

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Ottesen, T., Davis, K. C., Hobbs, L. K., Muncy, N. M., Stevens, N. M., Anderson, M., Johnson, P., Doxey, C. R., Richter, K., Wang, H., Hartley, R., Kirwan, C. B., Wisco, J. J.. 2020-09-03. Differences in Chemo-signaling Compound-Evoked Brain Activity in Male and Female Young Adults: A Pilot Study in the Role of Sexual Dimorphism in Olfactory Chemo-Signaling. https://doi.org/10.1101/2020.09.03.280685

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