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bioRxiv · 10.1101/2020.08.30.256743

Advanced eMAGE for highly efficient combinatorial editing of a stable genome

Liang, Z.·Metzner, E.·Isaacs, F. J.
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Abstract

Eukaryotic multiplex genome engineering (eMAGE) offers a powerful tool to generate precise combinatorial genome modifications in Saccharomyces cerevisiae. We optimize the design of synthetic oligonucleotides and enrichment of edited populations to increase editing frequencies up to 90%, reduce workflow time by 40%, and engineer a tunable mismatch repair system to lower the rate of spontaneous mutations [~]17-fold. These advances transiently evade genome maintenance to introduce multiple edits at high efficiencies in a stable genetic background, expanding utility of eMAGE in eukaryotic cells.

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Liang, Z., Metzner, E., Isaacs, F. J.. 2020-08-31. Advanced eMAGE for highly efficient combinatorial editing of a stable genome. https://doi.org/10.1101/2020.08.30.256743

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