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bioRxiv · 10.1101/2020.08.24.264580

An in vitro tumorigenesis model based on live cell-generated oxygen and nutrient gradients

Gilmore, A. C.·Flaherty, S. J.·Somasundaram, V.·Scheiblin, D. A.·Lockett, S. J.·Wink, D. A.·Heinz, W. F.
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Abstract

The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME and demonstrate that breast cancer cells and macrophages generate hypoxic and nutrient gradients, self-organize, and have spatially varying phenotypes along the gradients, leading to new insights into tumorigenesis.

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Gilmore, A. C., Flaherty, S. J., Somasundaram, V., Scheiblin, D. A., Lockett, S. J., Wink, D. A., Heinz, W. F.. 2020-08-25. An in vitro tumorigenesis model based on live cell-generated oxygen and nutrient gradients. https://doi.org/10.1101/2020.08.24.264580

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