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bioRxiv · 10.1101/2020.08.24.264069

Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses.

Abstract

B cell responses are a critical component of anti-viral immunity. However, a comprehensive picture of antigen-specific B cell responses, differentiation, clonal proliferation and dynamics in different organs after infection is lacking. Here, we combined single-cell RNA sequencing with single-cell B cell receptor (BCR) characterization of antigen-specific cells in the draining lymph nodes, spleen and lungs after influenza infection. We identify several novel B cell subpopulations forming after infection and find organ-specific differences that persist over the course of the response. We discover important transcriptional differences between memory cells in lungs and lymphoid organs and describe organ-restricted clonal expansion. Strikingly, by combining BCR mutational analysis, monoclonal antibody expression and affinity measurements we find no differences between germinal center (GC)-derived memory and plasmacells, at odds with an affinity-based selection model. By linking antigen-recognition with transcriptional programming, clonal-proliferation and differentiation, these finding provide important advances in our understanding of antiviral B cell immunity.

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BibTeXRIS

Mathew, N. R., Jayanthan, J. K., Smirnov, I., Robinson, J. L., Axelsson, H., Nakka, S. S., Emmanouilidi, A., Czarnewski, P., Yewdell, W. T., Lebrero-Fernandez, C., Bernasconi, V., Harandi, A. M., Lycke, N., Borcherding, N., Yewdell, J. W., Greiff, V., Bemark, M., Angeletti, D.. 2020-08-24. Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses.. https://doi.org/10.1101/2020.08.24.264069

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