Intercellular Arc signaling regulates vasodilation

Abstract

Injury responses require communication between different cell types in the skin. Sensory neurons contribute to inflammation and can secrete signaling molecules that affect non-neuronal cells. Despite the pervasive role of translational regulation in nociception, the contribution of activity-dependent protein synthesis to inflammation is not well understood. To address this problem, we examined the landscape of nascent translation in DRG neurons treated with inflammatory mediators using ribosome profiling. We identified the activity-dependent gene, Arc, as a target of privileged translation in vitro and in vivo. Inflammatory cues promote local translation of Arc in the skin. Arc deficient mice display exaggerated paw temperatures and vasodilation in response to an inflammatory challenge. Since Arc has recently been shown to be released from neurons in extracellular vesicles, we hypothesized that intercellular Arc signaling regulates the inflammatory response in skin. We found that the excessive thermal responses and vasodilation observed in Arc defective mice are rescued by injection of Arc-containing extracellular vesicles into the skin. Our findings suggest that activity-dependent production of Arc in afferent fibers regulates neurogenic inflammation through intercellular signaling. HIGHLIGHTSO_LIRibosome profiling identifies Arc as a target of activity-dependent translation C_LIO_LIArc is present in the DRG, spinal cord, and skin C_LIO_LIInduced Arc biosynthesis in skin requires the presence of afferent fibers C_LIO_LIArc-deficient mice have exaggerated inflammation that is rescued by Arc-containing extracellular vesicles C_LI