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bioRxiv · 10.1101/2020.08.12.247874

Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions

Bacon, K.·Blain, A.·Bowen, J.·Burroughs, M.·McArthur, N.·Menegatti, S.·Rao, B. M.
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Abstract

Quantifying the binding affinity of protein-protein interactions is important for elucidating connections within biochemical signaling pathways, as well as characterization of binding proteins isolated from combinatorial libraries. We describe a quantitative yeast-yeast two hybrid (qYY2H) system that not only enables discovery of specific protein-protein interactions, but also efficient, quantitative estimation of their binding affinities (KD). In qYY2H, the bait and prey proteins are expressed as yeast cell surface fusions using yeast surface display. We developed a semi-empirical framework for estimating the KD of monovalent bait-prey interactions, using measurements of the apparent KD of yeast-yeast binding, which is mediated by multivalent interactions between yeast-displayed bait and prey. Using qYY2H, we identified interaction partners of SMAD3 and the tandem WW domains of YAP from a cDNA library and characterized their binding affinities. Finally, we showed that qYY2H could also quantitatively evaluate binding interactions mediated by post-translational modifications on the bait protein.

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Bacon, K., Blain, A., Bowen, J., Burroughs, M., McArthur, N., Menegatti, S., Rao, B. M.. 2020-08-13. Quantitative yeast-yeast two hybrid for discovery and binding affinity estimation of protein-protein interactions. https://doi.org/10.1101/2020.08.12.247874

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