Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

Hussain, S. A.; Kubo, T.; Hall, N. J.; Gala, D.; Hampson, K.; Parton, R. M.; Phillips, M. A.; Wincott, M.; Fujita, K.; Davis, I.; Dobbie, I. M.; Booth, M. J.

doi:10.1101/2020.07.28.225607

bioRxiv · 10.1101/2020.07.28.225607

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

Hussain, S. A.·Kubo, T.·Hall, N. J.·Gala, D.·Hampson, K.·Parton, R. M.·Phillips, M. A.·Wincott, M.·Fujita, K.·Davis, I.·Dobbie, I. M.·Booth, M. J.

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Abstract

Adaptive optics is being applied widely to a range of microscopies in order to improve imaging quality in the presence of specimen-induced aberrations. We present here the first implementation of wavefront-sensorless adaptive optics for a laser-free, aperture correlation, spinning disk microscope. This widefield method provides confocal-like optical sectioning through use of a patterned disk in the illumination and detection paths. Like other high-resolution microscopes, its operation is compromised by aberrations due to refractive index mismatch and variations within the specimen. Correction of such aberrations shows improved signal level, contrast and resolution.

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Hussain, S. A., Kubo, T., Hall, N. J., Gala, D., Hampson, K., Parton, R. M., Phillips, M. A., Wincott, M., Fujita, K., Davis, I., Dobbie, I. M., Booth, M. J.. 2020-07-29. Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope. https://doi.org/10.1101/2020.07.28.225607

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