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bioRxiv · 10.1101/2020.07.28.224816

Regulation of specific cell clusters in TCR-T cells responding to differential expression of tumor PD-L1

Renpeng, D.·Liu, S.·Chen, H.·Kang, B.·Drmanac, R.·Gu, Y.·Dong, X.·Gao, Q.
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Abstract

PD-L1 signaling is important in regulating T cell function and keeping balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+ was upregulated after antigen stimulation and tumor PD-L1 modified TCR-T cell function through downregulating the percentages of clusters HLA-DR+CD28+CD8+ and HLA-DR+CD38+CD8+ which were higher in TCR-TMART-1 than in Tnull.

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Renpeng, D., Liu, S., Chen, H., Kang, B., Drmanac, R., Gu, Y., Dong, X., Gao, Q.. 2020-07-29. Regulation of specific cell clusters in TCR-T cells responding to differential expression of tumor PD-L1. https://doi.org/10.1101/2020.07.28.224816

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