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bioRxiv · 10.1101/2020.07.24.219493

Epidermal stratification is uncoupled from centrosome-dependent cell division orientation of the basal progenitors

Damen, M.·Soroka, E.·Khatif, H.·Kukat, C.·Simons, B. D.·Bazzi, H.
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Abstract

The development of complex stratified epithelial barriers in mammals is initiated from single-layered epithelia. How stratification is initiated and fueled are still open questions. Previous studies on skin epidermal stratification suggested a central role for perpendicular/asymmetric cell division orientation of the basal keratinocyte progenitors. Here, we use centrosomes, that organize the mitotic spindle, to test whether cell division orientation and stratification are linked. Genetically ablating centrosomes from the developing epidermis led to the activation of the p53-, 53BP1- and USP28-dependent mitotic surveillance pathway causing a thinner epidermis and hair follicle arrest. The centrosome/p53-double mutant keratinocyte progenitors significantly altered their division orientation in the later stages without majorly affecting epidermal differentiation. Together with time-lapse imaging and tissue growth dynamics measurements, the data suggested that the first and major phase of epidermal stratification is boosted by high proliferation rates of the basal and suprabasal transit-amplifying cells as well as cell delamination, whereas the second phase maybe uncoupled from the division orientation of the basal progenitors. The data provide insights for tissue homeostasis and hyperproliferative diseases that may recapitulate developmental programs.

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Damen, M., Soroka, E., Khatif, H., Kukat, C., Simons, B. D., Bazzi, H.. 2020-07-25. Epidermal stratification is uncoupled from centrosome-dependent cell division orientation of the basal progenitors. https://doi.org/10.1101/2020.07.24.219493

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