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bioRxiv · 10.1101/2020.07.23.218404

Bariatric surgery normalizes circulating glucocorticoid levels and lowers glucocorticoid action tissue-selectively in mice

Akalestou, E.·Lopez-Noriega, L.·Christakis, I.·Miras, A.·Leclerc, I.·Rutter, G. A.
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Abstract

BackgroundGlucocorticoids produced by the adrenal cortex are essential for the maintenance of metabolic homeostasis. Glucocorticoid activation is catalyzed by 11{beta}-hydroxysteroid dehydrogenase 1 (11{beta}-HSD1) and signalling is achieved through the glucocorticoid receptor (GR), a ligand-dependent transcription factor. Excess glucocorticoids are associated with insulin resistance and hyperglycaemia. A small number of studies have investigated the effects of bariatric surgery, a gastrointestinal procedure known to improve insulin sensitivity, on glucocorticoid metabolism, but the hypothesised mechanism is assumed to be via weight loss. AimTo investigate the effect of bariatric surgery on glucocorticoid metabolism in lean and obese mice. MethodsLean mice and HFD mice underwent Vertical Sleeve Gastrectomy (VSG) or sham surgery. Glucose and insulin tolerance tests were performed at four and ten weeks post operatively and circulating corticosterone was measured. Liver and adipose tissues were harvested from fed mice and 11{beta}-HSD1 and GR levels were measured by quantitative RT-PCR or Western (immuno-) blotting, respectively. ResultsVSG did not cause excess weight loss in lean mice when compared to sham operated mice. However, both lean and HFD VSG mice displayed significantly improved glucose clearance and insulin sensitivity. Remarkably, VSG restores physiological corticosterone production in HFD mice and reduces11{beta}-HSD1 levels at four and ten weeks post-surgery. Additionally, lean mice displayed significantly lowered mRNA levels of 11{beta}-HSD1 in subcutaneous adipose tissue and GR in liver. ConclusionsBariatric surgery improves insulin sensitivity and reduces glucocorticoid activation at tissular level, under physiological and pathophysiological (obesity) conditions, irrespective of weight loss. The reduction of glucocorticoid exposure may represent an additional contribution to the health benefits of bariatric surgery. These findings point towards a physiologically relevant gut-adrenal axis.

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Akalestou, E., Lopez-Noriega, L., Christakis, I., Miras, A., Leclerc, I., Rutter, G. A.. 2020-07-24. Bariatric surgery normalizes circulating glucocorticoid levels and lowers glucocorticoid action tissue-selectively in mice. https://doi.org/10.1101/2020.07.23.218404

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