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bioRxiv · 10.1101/2020.07.16.204636

Paracoccidioides genomes reflect high levels of species divergence and little interspecific gene flow

Abstract

The fungus Paracoccidioides spp. is a prevalent human pathogen endemic to South America. The genus is composed of five species. In this report, we use 37 whole genome sequences to study the allocation of genetic variation in Paracoccidioides. We tested three genome-wide predictions of advanced speciation, namely, that all species should be reciprocally monophyletic, that species pairs should be highly differentiated along the whole genome, and that there should be low rates of interspecific gene exchange. We find support for these three hypotheses. Species pairs with older divergences show no evidence of gene exchange, while more recently diverged species pairs show evidence of modest rates of introgression. Our results indicate that as divergence progresses, species boundaries become less porous among Paracoccidioides species. Our results suggest that species in Paracoccidioides are at different stages along the divergence continuum. IMPORTANCEParacoccidioides is the causal agent of the most frequent systemic mycosis in Latin America. Most of the inference of the evolutionary history of Paracoccidioides has used only a handful of molecular markers. In this report, we evaluate the extent of genome divergence among Paracoccidioides species and study the possibility of interspecific gene exchange. We find that all species are highly differentiated. We also find that the amount of gene flow between species is low and in some cases even completely absent in spite of geographic overlap. Our study constitutes a systematic effort to identify species boundaries in fungal pathogens, and determine the extent of gene exchange among fungal species.

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Mavengere, H., Mattox, K., Teixeira, M. M., Sepulveda, V., Gomez, O., Hernandez, O., McEwen-Ochoa, J. G., Matute, D. R.. 2020-07-17. Paracoccidioides genomes reflect high levels of species divergence and little interspecific gene flow. https://doi.org/10.1101/2020.07.16.204636

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