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bioRxiv · 10.1101/2020.07.15.204438

In situ generation of RNA complexes for synthetic molecular strand displacement circuits in autonomous systems

Bae, W.·Stan, G.-B. V.·Ouldridge, T. E.
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Abstract

Synthetic molecular circuits implementing DNA or RNA strand-displacement reactions can be used to build complex systems such as molecular computers and feedback control systems. Despite recent advances, application of nucleic acid-based circuits in vivo remains challenging due to a lack of efficient methods to produce their essential components - multi-stranded complexes known as "gates" - in situ, i.e. in living cells or other autonomous systems. Here, we propose the use of naturally occurring self-cleaving ribozymes to cut a single-stranded RNA transcript into a gate complex of shorter strands, thereby opening new possibilities for the autonomous and continuous production of RNA strands in a stoichiometrically and structurally controlled way.

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Bae, W., Stan, G.-B. V., Ouldridge, T. E.. 2020-07-15. In situ generation of RNA complexes for synthetic molecular strand displacement circuits in autonomous systems. https://doi.org/10.1101/2020.07.15.204438

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