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bioRxiv · 10.1101/2020.07.08.193672

Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus

Federico Cocozza·Ester Piovesana·Nathalie Névo·Xavier Lahaye·Julian Buchrieser·Olivier Schwartz·Nicolas Manel·Mercedes Tkach·Clotilde Théry·Lorena Martin-Jaular
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Abstract

ABSTRACTSARS-CoV-2 entry is mediated by binding of the spike protein (S) to the surface receptor ACE2 and subsequent priming by TMPRRS2 allowing membrane fusion. Here, we produced extracellular vesicles (EVs) exposing ACE2 and demonstrate that ACE2-EVs are efficient decoys for SARS-CoV-2 S protein-containing lentivirus. Reduction of infectivity positively correlates with the level of ACE2, is 500 to 1500 times more efficient than with soluble ACE2 and further enhanced by the inclusion of TMPRSS2.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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Federico Cocozza, Ester Piovesana, Nathalie Névo, Xavier Lahaye, Julian Buchrieser, Olivier Schwartz, Nicolas Manel, Mercedes Tkach, Clotilde Théry, Lorena Martin-Jaular. 2020-07-08. Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus. https://doi.org/10.1101/2020.07.08.193672

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