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bioRxiv · 10.1101/2020.07.01.181685

Transcription-dependent domain-scale 3D genome organization in dinoflagellates

Marinov, G. K.·Trevino, A. E.·Xiang, T.·Kundaje, A.·Grossman, A. R.·Greenleaf, W.
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Abstract

Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state, are not packaged by histones, and contain genes organized into polycistronic arrays, with minimal transcriptional regulation. We analyze the 3D genome of Breviolum minutum, and find large topological domains without chromatin loops, demarcated by convergent gene array boundaries ("dinoTADs). Transcriptional inhibition degrades dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates.

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Marinov, G. K., Trevino, A. E., Xiang, T., Kundaje, A., Grossman, A. R., Greenleaf, W.. 2020-07-02. Transcription-dependent domain-scale 3D genome organization in dinoflagellates. https://doi.org/10.1101/2020.07.01.181685

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