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bioRxiv · 10.1101/2020.06.29.178418

Consensus Finder web tool to predict stabilizing substitutions in proteins

Jones, B. J.·Kan, C. N. E.·Luo, C.·Kazlauskas, R.
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Abstract

The consensus sequence approach to predicting stabilizing substitutions in proteins rests on the notion that conserved amino acids are more likely to contribute to the stability of a protein fold than non-conserved amino acids. To implement a prediction for a target protein sequence, one finds homologous sequences and aligns them in a multiple sequence alignment. The sequence of the most frequently occurring amino acid at each position is the consensus sequence. Replacement of a rarely occurring amino acid in the target with a frequently occurring amino acid is predicted to be stabilizing. Consensus Finder is an open-source web tool that automates this prediction. This chapter reviews the rationale for the consensus sequence approach and explains the options for fine-tuning this approach using Staphylococcus nuclease A as an example.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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Jones, B. J., Kan, C. N. E., Luo, C., Kazlauskas, R.. 2020-06-30. Consensus Finder web tool to predict stabilizing substitutions in proteins. https://doi.org/10.1101/2020.06.29.178418

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